Antigen

Antigen Class

TFs and others

Antigen

Kmt2d

Cell type

Cell type Class

Neural

Cell type

HT22

NA

NA

Sample

source_name

HT22 hippocampal cell line

sample type

wild-type clone

genotype

wild-type

tissue

hippocampal cell line

cell line

HT22

chip antibody

KMT2D (Millipore ABE1867)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclear lysates were pre-cleared and histone-DNA complexes were isolated with anti-KMT2D antibody (Millipore ABE1867) and purified by DNA Clean and Concentrator (Zymo Research) NEBNext UltraII DNA Library Prep Kit for Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

24538097

Reads aligned (%)

93.1

Duplicates removed (%)

7.5

Number of peaks

2854 (qval < 1E-05)

mm9

Number of total reads

24538097

Reads aligned (%)

92.9

Duplicates removed (%)

8.2

Number of peaks

2857 (qval < 1E-05)