Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kmt2d

Cell type

Cell type Class
Neural
Cell type
HT22
NA
NA

Attributes by original data submitter

Sample

source_name
HT22 hippocampal cell line
sample type
wild-type clone
genotype
wild-type
tissue
hippocampal cell line
cell line
HT22
chip antibody
KMT2D (Millipore ABE1867)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclear lysates were pre-cleared and histone-DNA complexes were isolated with anti-KMT2D antibody (Millipore ABE1867) and purified by DNA Clean and Concentrator (Zymo Research) NEBNext UltraII DNA Library Prep Kit for Illumina

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
24538097
Reads aligned (%)
93.1
Duplicates removed (%)
7.5
Number of peaks
2854 (qval < 1E-05)

mm9

Number of total reads
24538097
Reads aligned (%)
92.9
Duplicates removed (%)
8.2
Number of peaks
2857 (qval < 1E-05)

Base call quality data from DBCLS SRA